The Exogenous Estrogen Antiproliferaive Effects On Cervical Cancer Cell Line: An In Vitro Study

Document Type: Original Article


1 Department of Biotechnology, Faculty of Advanced Sciences & Technology. Pharmaceutical Sciences Branch, Islamic Azad University, Tehran-Iran (IAUPS)

2 Department of Physiology, Faculty of Basic Sciences, Islamic Azad University, Hamedan Branch, Hamedan, Iran


Estradiol valerate (Exogenous estrogen) is a synthetic ester and is also a female estrogen hormone. There are
studies showing that estradiol may have anticancer effects. This study was performed to reveal the effects of
exogenous estrogens on the viability of cervical cancer cells in cell culture. We used Hela cells as our cell line
in this study. Hela cells were exposed to 0.0001, 0.001, 0.01, 0.1, 1 and 10 mg/ml of estradiol. Control Hela
cells were not exposed to estradiol. MTT assay was used to determine the viability of cervical cancer cells in
cell culture. Results indicated that administration of 10 mg/ml Estradiol led to significant decrease in viability
of Hela cells compared to control cells (P<0.05). Administration of 0.0001, 0.001, 0.01, 0.1 and 1 mg/ml of
estradiol did not change the viability of Hela cells significantly compared to control group. The results are
expressed as the mean ± SD of more than 3 independently performed experiments. Statistical significance was
set at pcell growth rate, According to the findings, high doses of estradiol have cytotoxic effects on cervical cancer
cells. There are no studies revealing that estradiol may reduce the viability of cervical cancer cells.


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