Study of microbial contamination of hospital antiseptics and disinfectants

Document Type: Original Article


Nowadays, the prevalence of nosocomial infections are considered as a serious problem in health systems.
Use of appropriate antiseptics and disinfectants play major role in the control of nosocomial infections.
In this study, we examined microbial contamination of hospital antiseptics and disinfectants and the effect
of dilution on their rate of contamination to gain insight into their rational use in controlling nosocomial
Samples from three types of antiseptics and disinfectants, each from 2 different brands, were prepared (A
& B). Hand samples were prepared in the form of solution and were sampled for microbial control after
removing the cap. Instrument and surface samples were in concentrated form and dilutable, that were tested
in four different times. The mentioned samples were assessed by plate count method and membrane filtration.
We also assessed the efficacy of the mentioned samples by adding 106 microorganisms per ml to six different
genera, including S. aureus, P.aeruginosa, E. coli, B. subtilis, C. albicans, A. niger. After passing the golden
time the samples were cultured. The bacterial and fungal count tests, the sterility tests and the efficacy tests
of mentioned samples showed no contamination