Effect of Coenzyme Q10 (ubiquinone) on hippocampal CA1 pyramidal cells following transient global ischemia/reperfusion in male wistar rat

Document Type: Original Article


Ischemia/Reperfusion (I/R)-induced cerebral injury has been reported as a leading cause of death
and long-term disabilities. Hippocampus is an area which is more sensitive to be affected by I/R
and hypoxic conditions. Coenzyme Q10 is a strong antioxidant which plays a role in membrane
stabilization. This study aims to investigate the possible role of CoQ10 in ameliorating the
histomorphological changes in the hippocampal CA1 pyramidal cells induced by cerebral I/R.
Thirty six adult male wistar rats were divided into six groups, each group consisting of six
rats, including control, ischemia, vehicle and CoQ10-treated (10, 50,100 mg/kg). In treatment
groups, rats were orally administered CoQ10 during five days before and three days after I/R.
Global cerebral ischemia was induced by bilateral common carotid arteries occlusion for about
20 minutes, followed by reperfusion. H&E and Nissl staining were utilized for some qualitative
and quantitative studies. Then the histomorphological changes were measured by Image Tools
2 software.
The data analysis showed a significant reduction in the number of CA1 pyramidal cells after I/R;
whearas, no significant difference was seen in the number of cells in mentioned region between
control and 100 mg/kg CoQ10-treated groups.
The findings indicate the neurotrophic properties of CoQ10 and support the beneficial effects of
CoQ10 as an adjuvant therapy in patients who have risk factor(s) of ischemic stroke.