Document Type: Original Article
Antibiotic resistance is an emerging problem worldwide present in many bacteria, specially
S. aureus, P. aeroginosa, and E. coli that are consider as the most common group of bacteria
responsible for nosocomial infections. This problem would be more serious and divesting in
developing countries where there is not regular surveillance program for periodic antibiotic
resistance. In attempts to report the antibiotic pattern in Iranian cities, we collected clinical
isolates and tested them against current antibiotics. 653 Clinical isolates of S. aureus, P. aeroginosa,
and E. coli were collected during 5 months. The isolates were then transported to research
laboratory within glycerol containing medium. Standard antibiotic discs containing different
concentrations of each agent were provided from MAST media. Standard disc diffusion method
was carried out and the result was interpreted using NCCLS tables and data charts. Isolates
were mostly cultured from respiratory, Urine, wounds, blood clinical samples. The clinical
isolates were E. coli (45%) S. aureus (33%), and P. aeroginosa (22%). 20% of E. coli, 9% of P.
aeroginosa, and 12% of S. aureus isolates showed reduction of sensitivity to Amikacin (AK).
27% of S. aureus had reduction of sensitivity to Vancomycin (VA). About 25% of P. aeroginosa
showed reduced sensitivity to Gentamicin (GM).